aggregation of vesicles
Encapsulation of Tetraphenylethylene Derivative in Liposome Vesicles …
In this work, a sensitive signal-on electrochemiluminescence biosensor using liposome-encapsuled 1,1,2,2-tetra(4-carboxylphenyl)ethylene (TPE) as a promising aggregation-induced electrochemiluminescence (AIECL) emitter for detection of biomarkers was developed. Aggregation-induced enhancement occurs …
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Aggregation of acidic lipid vesicles by myelin basic protein …
Therefore, the effect of K+ concentration on the ability of MBP to aggregate large unilamellar vesicles (LUVs) containing phosphatidylcholine (PC) and 10-20% acidic lipid was investigated. At MBP to lipid ratios where there was an excess of acidic lipid, physiological increases in K+ concentration up to about 100 mM greatly increased MBP ...
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Extracellular vesicle analysis | Nature Reviews Methods Primers
Cells release small, phospholipid membrane-enclosed particles, collectively referred to as extracellular vesicles (EVs), into their surroundings to enable intercellular …
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High-speed centrifugation induces aggregation of extracellular vesicles
Aggregation of vesicles results in the observation of multiple particles at a single time which results in inaccurate data . Thus, it requires the immobilization of …
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Cooperative aggregation of gold nanoparticles on phospholipid vesicles
Our observations reveal a strong influence of electrolyte concentration on AuNP aggregation mediated by vesicles. Notably, cluster formation is observed even at less than one AuNP per vesicle ratio at low enough ionic strengths. These results evidence a binding mechanism governed by electrostatic attraction with a distinct cooperative …
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Brain metastases-derived extracellular vesicles induce …
Brain metastases-derived extracellular vesicles induce binding and aggregation of low-density lipoprotein J Nanobiotechnology. 2020 Nov 7 ... Specifically, Br-EVs trigger LDL aggregation, and the presence of LDL accelerates Br-EV uptake by monocytes, which are key components in the brain metastatic niche.
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Composition and functions of bacterial membrane vesicles
Extracellular vesicles are produced by species across all domains of life, suggesting that vesiculation is a fundamental process of living matter 1.Bacterial membrane vesicles (MVs), which are ...
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Biological surface properties in extracellular …
Extracellular membrane vesicles (EVs) from biological fluids have been proposed as a promising source of protein biomarkers …
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Histology, Extracellular Vesicles
Extracellular vesicles (EVs) are a cluster of nano-sized vesicles of different sizes, cargo, and surface markers that are secreted into the extracellular environment through a variety of mechanisms. They carry various components of the cytoplasm and cell membrane that are selectively loaded into these vesicles. They are …
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High-speed centrifugation induces aggregation of …
We show here, using electron microscopy, receptor-specific gold labelling and flow cytometry, that high-speed centrifugation induces the formation of EVaggregates …
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Sphingosine induces the aggregation of imine-containing
Our results show that sphingosine causes aggregation of Cu(2+)-peroxidized vesicles. We observed that aggregation is facilitated by the presence of negatively-charged phospholipids in the membrane, and inhibited by anti-oxidants e.g. BHT. Interestingly, long-chain alkylamines (C18, C16) but not their short-chain analogues (C10, C6, C1) can ...
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Deciphering Ethanol-Driven Swelling, Rupturing, Aggregation…
The supported model bilayer was composed of 1,2-dioleoyl-sn-phosphatidylcholine (DOPC), cholesterol, and sphingomyelin, and the bilayers were formed by vesicle fusion from extruded unilamellar ...
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In Vivo Real-Time Imaging of Extracellular Vesicles in Liver
Extracellular vesicles (EVs) attract much attention in liver pathology because they regulate cell-cell communication and many pathophysiological events by transferring their cargos. ... Herein, we demonstrate the use of an aggregation-induced emission luminogen, DPA-SCP, for the real-time tracking of EVs derived from human …
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Tubulation and aggregation of spherical nanoparticles adsorbed on vesicles
The membrane tubules enclose linear aggregates of particles and protrude into the vesicles. The high stability of the particle-filled tubules implies strongly attractive, membrane-mediated interactions between the particles. The tubular structures may provide a new route to encapsulate nanoparticles reversibly in vesicles.
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Glucocerebrosidase deficiency promotes protein aggregation …
Mutations in the glucosylceramidase beta (GBA) gene are strongly associated with neurodegenerative diseases marked by protein aggregation. GBA encodes the lysosomal enzyme glucocerebrosidase, which breaks down glucosylceramide. A common explanation for the link between GBA mutations and protein aggr …
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Lysophagy protects against propagation of α-synuclein aggregation …
The neuron-to-neuron propagation of misfolded α-synuclein (αSyn) aggregates is thought to be key to the pathogenesis of synucleinopathies. Recent studies have shown that extracellular αSyn aggregates taken up by the endosomal–lysosomal system can rupture the lysosomal vesicular membrane; however, it remains unclear …
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Annexin A1–dependent tethering promotes …
Single-EV microarray and proteomics revealed increased ANXA1 primarily on aggregating and calcifying microvesicles. ANXA1 vesicle aggregation was suppressed by calcium chelation, altering pH, or ANXA1 …
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Composition and functions of bacterial membrane vesicles
This extensive study demonstrates that extraintestinal pathogenic E. coli (ExPEC) produces at least three types of vesicle: OMVs, OIMVs and EOMVs.
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Stepwise aggregation of dimethyl-di-n-octylammonium …
The self-aggregation of dimethyl-di-n-octylammonium chloride, in diluted aqueous solutions, was studied with various experimental and theoretical techniques: zetametry, conductimetry, dimethyl-di-n-octylammonium and chloride-selective electrodes, tensiometry, NMR spectroscopy ((1)H and DOSY), and molecular modeling (PM3 and …
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In Vivo Real-Time Imaging of Extracellular Vesicles in Liver
Extracellular vesicles (EVs) attract much attention in liver pathology because they regulate cell–cell communication and many pathophysiological events by transferring their cargos. Monitoring and understanding the in vivo fate and therapeutic capacity of these EVs is critical for the development and optimization of EV-based diagnosis and therapy. …
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Overview of Extracellular Vesicles, Their Origin, Composition, Purpose
Aggregation of vesicles results in the observation of multiple particles at a single time which results in inaccurate data . Thus, it requires the immobilization of exosomes on the surface of beads (either by immunocapture or covalent conjugation) in order to be observed by the flow cytometer. Once ...
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Aggregation and fusion of phospholipid vesicles
Monovalent cations induce reversible aggregation of negatively charged vesicles, but they inhibit the fusion induced by divalent cations such as Ca 2+ or Mg 2+. Fusion of neutral phospholipid vesicles, and it occurs the cation-induced fusion of acidic phospholipid vesicles, and it occurs only at temperatures below the gel to liquid …
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High-speed centrifugation induces aggregation of …
vesicles that are released by cells upon activation or apoptosis (1 3). According to current hypo-theses, cell-derived vesicles either form by blebbing and shedding of plasma membrane pieces or they are secreted by cells after fusion of multivesicular endosomes with the plasma membrane. Here, the term extracellular vesicle
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Self-limiting aggregation of phospholipid vesicles
Vesicle aggregation experiments using cationic polymers were performed in a similar way to those with biotin and streptavidin: we started with single vesicles and stepwise added linker molecules to induce aggregation. The linker molecule here is the polycation poly-L-lysine (PLL). We continued the addition of PLL until unlimited …
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Lysophagy protects against propagation of α-synuclein aggregation …
The neuron-to-neuron propagation of misfolded α-synuclein (αSyn) aggregates is thought to be key to the pathogenesis of synucleinopathies. Recent studies have shown that extracellular αSyn aggregates taken up by the endosomal–lysosomal system can rupture the lysosomal vesicular membrane; however, it remains unclear whether lysosomal …
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Directed aggregation and fusion of lipid vesicles induced by …
We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affec …
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Phospholipid vesicle aggregation: effect of monovalent …
Interactions of lysozyme with phospholipid vesicles: effects of vesicle biophysical features on protein misfolding and aggregation. Soft Matter 2012, 8 (35), 9115.
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Aggregation of bacillus thuringiensis Cry1A toxins upon …
When bound to vesicles from the midguts of Manduca sexta larvae, the Cry1Ac toxin was largely resistant to digestion with protease K. ... In both cases, most of the toxin formed a large, antigenic aggregate of ca. 200 kDa. These toxin aggregates did not include the toxin receptor aminopeptidase N, but interactions with other vesicle components ...
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Induced fusion and aggregation of bacterial outer membrane vesicles …
Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion. Electrolytes and low pHs induced OMV aggregation for both native and modified LPS …
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Temperature-Promoted Giant Unilamellar Vesicle (GUV) Aggregation…
The evolution of unicellular to multicellular life is considered to be an important step in the origin of life, and it is crucial to study the influence of environmental factors on this process through cell models in the laboratory. In this paper, we used giant unilamellar vesicles (GUVs) as a cell …
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